AccuTarget™ Human Premade siRNA Set

합성된 모든 siRNA은 MALDI-TOF mass spectrometry를 통해 품질이 엄격히 제어되며 PAGE분석을 통해 duplex structure를 확인합니다 (Figure. 1, 2).

Figure 1. MALDI-TOF mass spectrometry analysis of a custom siRNA. All siRNAs are processed by MALDI-TOF mass spectrometry to ensure its quality.

Figure 2. PAGE data of double-stranded custom siRNA. Complementary single-stranded RNA strands were hybridized to form siRNA duplex and analyzed by 15% non-denaturing PAGE.
- SS: single-stranded RNA
- DS: double-stranded siRNA

Figure 3. Knockdown efficiency of AccuTarget ™ siRNA Library. To determine the knockdown efficiency, HeLa cells were transfected with individual siRNAs at 100 nM concentration. 24 hours post-transfection, total RNA was isolated and the level of target mRNA was measured by qRT-PCR. This data demonstrates the effectiveness of the Turbo si-Designer algorithm: 83.8% of tested siRNAs induced > 70% knockdown and 38.1% of tested siRNAs elicited > 90% knockdown.

Figure 4. Knockdown efficiency of AccuTarget ™ Human Validated Cell Cycle siRNA set. HeLa cells were transfected with individual siRNAs targeting 33 different genes at a concentration of 20 nM. Total RNAs was isolated and the level of target mRNA was measured by qRT-PCR. This data demonstrates the effectiveness of the Turbo si-Designer algorithm.

바이오니아는 즉시 실험에 사용할 수 있도록 제작된 25,368 개의 siRNA로 이루어진 AccuTarget ™ Premade Human siRNA Library를 제공합니다. 이러한 Premade siRNA Library는 10, 20, 50또는 100 nmole Guaranteed yield 로 주문하실 수 있습니다. 다양한 Cellular Processes, 암, 질병 관련 연구목적의 25개 Pathway별 siRNA Library set를 제공합니다. 이러한 siRNA는 0.1, 0.25, 0.5, 1 nmole Guaranteed Yield 로 주문할 수 있습니다. 또한 높은 Knockdown 효능이 검증된 21개의 Pre-validated Library를 제공합니다. Pre-validated siRNA Library는 10, 20, 50, 또는 100 nmole Guaranteed Yield로 주문하실 수 있습니다.

▶ siRNA Set

Bioneer는 다양한 Cellular Processes, 암, 질병관련 연구목적의 25개 Gene Family 및 Pathway별로 siRNA Library Set를 제공합니다. 이러한 siRNA는 0.1, 0.25, 0.5, 1 nmole Guaranteed Yield 로 주문하실 수 있습니다.

▶ siRNA Subset

siRNA subset library는 Kinase 또는 Phosphatase library의 functional family subset입니다 .이러한 siRNA는 0.1, 0.25, 0.5, 1 nmole guaranteed yield 로 주문하실 수 있습니다.

▶ siRNA Druggable Set

0.1, 0.25, 0.5, 1 nmole Guaranteed Yield 로 주문하실 수 있습니다.