특장점
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핵산 추출에 필요한 enzyme 및 시약이 분주된 pre-filled buffer cartridge system 사용
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다양한 식물 시료에 최적화된 Plant Lysis Buffer 및 소모품류 (disposable filter tip, elution tube 등) 제공
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바이오니아의 독자적 기술로 개발, 생산된 실리카 자성입자를 사용하여 고순도, 고수율의 핵산 추출
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전자동 핵산 추출장비 (ExiPrep™ 16 Plus, ExiProgen™)를 이용하는 제품으로 재현성 있는 결과를 얻을 수 있음
응용 분야
• PCR
• Quantitative real-time PCR
• Gene cloning
• Genetics
• GMO detection
▶ 제품 규격/사양
| Feature |
Specification |
| Starting material |
up to 50 mg (seed), up to 100 mg (leaf) |
| Elution volume |
50-100 μl |
| Expected yield |
0.25-5 μg |
| Expected purity |
A260/280: > 1.7 |
▶ 구성품
| 구성품 |
Quantity |
Note |
| Buffer Cartridge ① |
6 ea |
|
| Buffer Cartridge ② |
6 ea |
|
| Seed Lysis Buffer |
1 ea |
30 ml |
| Precipitation Buffer |
1 ea |
10 ml |
| Proteinase K |
2 ea |
20 mg/tube |
| RNase A |
2 ea |
24 mg/tube |
| Disposable filter tip |
3 pack |
32 ea/pack |
| Elution tube |
1 pack |
12 ea/pack |
| 사용설명서 |
1 ea |
|
▶ 실험 자료
Figure 1. Comparison of genomic DNA extracted from Citrullus lanatus seed (50 mg) using ExiPrep™ Plus Seed Genomic DNA Kit (Bioneer, automatic), ExiPrep™ Plus Plant Genomic DNA Kit (Bioneer, automatic), MagListo™ 5M Plant Genomic DNA Extraction Kit (Bioneer, manual/magnetic bead) and a competitor’s kit (Competitor Q, manual/single column).
M: Bioneer 1 kb ladder
1-2: Bioneer ExiPrep™ Plus Seed Genomic DNA Kit, mean yield; 2.8 μg, mean purity; 1.74
3-4: Bioneer ExiPrep™ Plus Plant Genomic DNA Kit, mean yield; 1.9 μg, mean purity; 1.4
5-6: Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit, mean yield; 3.4 μg, mean purity; 1.66
7-8: Competitor Q Genomic DNA Extraction Kit, mean yield; 2.1 μg, mean purity; 1.5
Figure 2. Comparison of genomic DNA extracted from Zea mays seed (50 mg) using ExiPrep™ Plus Seed Genomic DNA Kit (Bioneer, automatic), ExiPrep™ Plus Plant Genomic DNA Kit (Bioneer, automatic), MagListo™ 5M Plant Genomic DNA Extraction Kit (Bioneer, manual/magnetic bead) and a competitor’s kit (Competitor Q, manual/single column).
M: Bioneer 1 kb ladder
1-2: Bioneer ExiPrep ™ Plus Seed Genomic DNA Kit, mean yield; 3.7 μg, mean purity; 1.70
3-4: Bioneer ExiPrep™ Plus Plant Genomic DNA Kit, mean yield; 1.6 μg, mean purity; 2.1
5-6: Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit, mean yield; 2.7 μg, mean purity; 1.67
7-8: Competitor Q Genomic DNA Extraction Kit, mean yield; 0.9 μg, mean purity; 1.91
Figure 3. Comparison of genomic DNA extracted from Callistephus chinensis seed (50 mg) using ExiPrep™ Plus Seed Genomic DNA Kit (Bioneer, automatic), ExiPrep™ Plus Plant Genomic DNA Kit (Bioneer, automatic), and MagListo™ 5M Plant Genomic DNA Extraction Kit (Bioneer, manual/magnetic bead).
M: Bioneer 1 kb ladder
1-2: Bioneer ExiPrep™ Plus Seed Genomic DNA Kit, mean yield; 4.4 μg, mean purity; 1.58
3-4: Bioneer ExiPrep™ Plus Plant Genomic DNA Kit, mean yield; 4.7 μg, mean purity; 1.66
5-6: Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit, mean yield; 5.2 μg, mean purity; 1.61
Figure 4. Comparison of Conventional PCR targeting sus1 (sucrose synthase 1) gene from Maize genomic DNA purified with ExiPrep™ Plus Seed Genomic DNA Kit (Bioneer, automatic), MagListo™ 5M Plant Genomic DNA Extraction Kit (Bioneer, manual/magnetic bead) and competitor’s kit (Competitor Q, single column).
M: Bioneer 100bp plus ladder
1-2: PCR product from genomic DNA of 1 mg seed purified with ExiPrep™ Plus Seed Genomic DNA Kit
3-4: PCR product from genomic DNA of 5 mg seed purified with ExiPrep™ Plus Seed Genomic DNA Kit
5-6: PCR product from genomic DNA of 10 mg seed purified with ExiPrep™ Plus Seed Genomic DNA Kit
7-8: PCR product from genomic DNA of 50 mg seed purified with ExiPrep™ Plus Seed Genomic DNA Kit
9-10: PCR product from genomic DNA of 50 mg seed purified with MagListo™ 5M Plant Genomic DNA Extraction Kit
11-12: PCR product from genomic DNA of 50 mg seed purified with competitor Q kit
13-14: Negative control
