▶ 제품 규격/사양
Feature |
Specification |
Sample type |
Various kind of forensic sample |
Technology |
Magnetic nano bead |
Hands-on time |
< 10 min |
Expected purity |
A260/280 > 1.8 |
* 시료의 종류에 따라 측정 값의 차가 있을 수 있습니다.
▶ 구성품
구성품 |
K-3615 |
TL Buffer (Lysis) |
35 ml x 1 ea |
GB Buffer (Binding) |
30 ml x 1 ea |
WM1 Buffer (1st Washing) |
40 ml x 1 ea |
W2 Buffer (2nd Washing) |
80 ml x 1 ea |
WE Buffer (3rd Washing) |
80 ml x 1 ea |
EA Buffer (Elution) |
15 ml x 1 ea |
Poly (A), lyophilized |
1 mg x 1 ea |
Proteinase K, lyophilized |
25 mg x 2 ea |
Magnetic Nano Bead - DNA |
1.8 ml x 6 ea |
사용설명서 |
1 ea |
▶ 실험 자료
(A) Urine
Product |
Sample volume |
10 ml |
5 ml |
1 ml |
NTC |
MagListo™ |
32.79 |
33.53 |
35.24 |
product |
32.33 |
33.08 |
35.21 |
Q |
32.48 |
33.36 |
38.93 |
32.76 |
33.08 |
40.16 |
(B) Saliva & Blood
Saliva |
Kit |
Blood |
Kit |
MagListo™ |
Q |
MagListo™ |
1 µl |
33.98 |
33.40 |
1 µl |
31.42 |
33.66 |
33.51 |
31.32 |
10 µl |
30.55 |
30.93 |
10 µl |
28.32 |
30.44 |
31.28 |
28.14 |
100 µl |
26.91 |
30.93 |
100 µl |
28.32 |
27.66 |
27.64 |
25.51 |
NTC |
U.D. |
U.D. |
(C) Saliva (10 ul) & Blood (10 ul) stains from clothes (7 mm diameter)
Sample |
Kit |
MagListo™ |
Q |
Saliva |
30.30 |
32.05 |
30.60 |
32.14 |
Blood |
29.15 |
29.69 |
29.14 |
29.89 |
NTC |
U.D. |
U.D. |
(D) Saliva (10 ul) and fingerprint on paper (7 mm diameter)
Sample |
Kit |
MagListo™ |
Q |
Saliva |
28.07 |
28.1 |
28.43 |
28.59 |
Fingerprint |
36.33 |
36.45 |
36.29 |
36.45 |
NTC |
U.D. |
U.D. |
(E) Saliva on cotton swab and chewing gum
Kit |
Ct |
Swab |
Gum |
MagListo™ |
26.41 |
32.10 |
28.29 |
32.25 |
Q |
26.87 |
31.21 |
26.45 |
31.10 |
NTC |
U.D. |
U.D. |
(F) Hair 1 strand (25 cm)
Sample |
Kit |
MagListo™ |
Q |
w/ root |
29.99 |
30.7 |
30.04 |
30.4 |
w/o root |
33.39 |
34.66 |
33.08 |
34.22 |
NTC |
U.D. |
U.D. |
(G) Nail clipping (15 mg)
Kit |
Ct |
MagListo™ |
28.00 |
27.98 |
Q |
30.04 |
29.46 |
NTC |
U.D. |
U.D. |
Figure 1. Comparison of real-time PCR data of DNA extracted using MagListo™ and competitor’s product (single-column type).
Figure 2. Comparison of total DNA from skeletal muscle tissue purified with MagListo™ and competitor’s kit (single column type).
M; 1 kb Ladder
1 mg
MagListo™; yield: 1.35 ug purity(A260/280): 1.95
Competitor; yield: 0.57 ug purity(A260/280): 1.86
5 mg
MagListo™; yield: 5.22 ug purity(A260/280): 1.90
Competitor; yield: 2.81 ug purity(A260/280): 1.88
Figure 3. Comparison of DNA extraction time from forensic sample purified with MagListo™ and competitor’s kit (single column type).
N: Number of Samples