▶ 제품 규격/사양
| Scale |
Mini
(typical yield (μg)) |
Midi
(typical yield (μg)) |
Maxi
(typical yield (μg)) |
| Plant tissue |
Up to 100 mg |
Up to 500 mg |
Up to 1 g |
| Seed |
Up to 50 mg |
Up to 250 mg |
Up to 500 mg |
| Elution volume |
100 ul |
500 ul |
1 ml |
| Typical DNA yield* |
5 - 15 ug |
15 - 80 ug |
30 - 150 ug |
| Expected purity |
A260/280 > 1.7 |
* 시료의 종류에 따라 측정 값의 차가 있을 수 있습니다.
▶ 구성품
| 구성품 |
K-3605* |
| PL Buffer |
35 ml x 1 ea |
| PC Buffer |
30 ml x 1 ea |
| PWM1 Buffer |
60 ml x 1 ea |
| W2 Buffer |
80 ml x 1 ea |
| WE Buffer |
120 ml x 1ea |
| EA Buffer |
25 ml x 1 ea |
| RNase A powder, lyophilized |
24 mg x 2 ea |
| Proteinase K, lyophilized |
25 mg x 2 ea |
| Magnetic Nanobead |
11 ml x 1 ea |
| 사용설명서 |
1 ea |
*mini-100 rxn, midi-15 rxn, maxi-8 rxn
Figure 1. Comparison of genomic DNA from plant purified with MagListo™ 5M Plant Genomic DNA Extraction Kit and competitor’s kit (single column type).
- M; Bioneer 1 kb ladder
- B; Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit
- Q; Competitor Q kit
Perilla; Genomic DNA from 100 mg leaves of Perilla frutescens var. japonica Regel
B; mean yield; 7.1 μg, mean purity; 1.80 Q; mean yield; 3.5 μg, mean purity; 1.85
Chinese cabbage; Genomic DNA from 100 mg leaves of Brassica rapa var. glabra Regel
B; mean yield; 5.9 μg, mean purity; 1.82 Q; mean yield; 4.7 μg, mean purity; 1.82
Zucchini; Genomic DNA from 100 mg tissue of Cucurbita pepo
B; mean yield; 3.6 μg, mean purity; 1.77 Q; mean yield; 3.0 μg, mean purity; 1.79
Onion; Genomic DNA from 100 mg tissue of Allium cepa
B; mean yield; 5.5 μg, mean purity; 1.80 Q; mean yield; 1.6 μg, mean purity; 1.84
Kidney bean; Genomic DNA from 50 mg seeds of Phaseolus vulgrais
B; mean yield; 7.7 μg, mean purity; 1.73 Q; mean yield; 6.6 μg, mean purity; 1.80
Figure 2. Comparison of genomic DNA from Brassica oleracea var. italic (broccoli) tissue purified with MagListo™ 5M Plant Genomic DNA Extraction Kit and competitor’s kit (single column type).
M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from broccoli (100 mg) purified with Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit, mean yield; 4.7 μg, mean purity; 1.85
3-4; (Mini)Genomic DNA broccoli (100mg) purified with competitor Q kit, mean yield; 3.7 μg, mean purity; 1.84
5-6; (Midi)Genomic DNA from broccoli (500mg), mean yield; 25.9 μg, mean purity; 1.86
7-8; (Maxi)Genomic DNA from broccoli (1g), mean yield; 48.2 μg, mean purity; 1.84
Figure 3. Comparison of genomic DNA from Cucumis sativus (cucumber) seed purified with MagListo™ 5M Plant Genomic DNA Extraction Kit and competitor’s kit (single column type).
M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from cucumber seed (50 mg) purified with Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit, mean yield; 2.8 μg, mean purity; 1.68
3-4; (Mini)Genomic DNA from cucumber seed (50 mg) purified with competitor Q kit, mean yield; 1.9 μg, mean purity; 1.83
5-6; (Midi)Genomic DNA from cucumber seed (250 mg), mean yield; 12.45 μg, mean purity; 1.69
7; (Maxi) Genomic DNA from cucumber seed (500 mg), yield; 20.8 μg, purity; 1.71
Figure 4. Electrophoresis data of conventional PCR of plant genomic DNA purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).
M; Bioneer 100 bp ladder
1-2; 100 bp fragment of EMBRYONIC FLOWER 2 (EMF2) gene from broccoli genomic DNA purified with Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit
3-4; 100 bp fragment of EMBRYONIC FLOWER 2 (EMF2) gene from broccoli genomic DNA purified with competitor Q kit
5-6; 180 bp fragment of glyceraldehyde-3-phosphate dehydrogenase A (GAPDH) gene from cucumber seed genomic DNA purified with Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit
7-8; 180 bp fragment of glyceraldehyde-3-phosphate dehydrogenase A (GAPDH) gene from cucumber genomic DNA purified with competitor Q kit
