▶ 제품 규격/사양
| Scale |
Mini
(typical yield (μg)) |
Midi
(typical yield (μg)) |
Maxi
(typical yield (μg)) |
| Whole blood |
200 μl (< 10 μg) |
2 ml (< 80 μg) |
4 ml (< 150 μg) |
| Cultured cell |
Up to 1 x 106 (< 12 μg) |
Up to 5 x 106 (< 60 μg) |
Up to 1 x 107 (< 120 μg) |
| Animal tissue |
Up to 25 mg (< 10 μg) |
Up to 100 mg (< 40 μg) |
Up to 250 mg (< 120 μg) |
| Bacteria (Gram (-), (+)) |
Up to 1 x 109 (< 15 μg) |
Up to 5 x 109 (< 80 μg) |
Up to 1 x 1010 (< 150 μg) |
| Expected purity |
A260/280 > 1.8 |
* 시료의 종류에 따라 측정 값의 차가 있을 수 있습니다.
▶ 구성품
| 구성품 |
K-3603* |
| TL Buffer |
35 ml x 1 ea |
| GB Buffer |
30 ml x 1 ea |
| WM1 Buffer |
60 ml x 1 ea |
| W2 Buffer |
80 ml x 1 ea |
| WE Buffer |
120 ml x 1 ea |
| EA Buffer |
25 ml x 1 ea |
| RNase A powder, lyophilized |
24 mg x 2 ea |
| Proteinase K, lyophilized |
25 mg x 2 ea |
| Magnetic Nanobead |
11 ml x 1 ea |
| 사용설명서 |
1 ea |
* mini-100 rxn, midi-15 rxn, maxi-7 rxn
▶ 실험 자료
 |
M; Bioneer 1 kb ladder
1-4; Blood genomic DNA purified with Bioneer MagListo™ 5M Genomic DNA Extraction Kit, mean yield; 6.45 μg, mean purity; 1.85
5-7; Blood genomic DNA purified with competitor Q kit, mean yield; 5.53 μg, mean purity; 1.85
|
Figure 1. Comparison of genomic DNA from human whole blood (200 μl) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).
 |
M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from 100 μl of blood, mean yield; 3 μg, mean purity; 1.80
3-6; (Midi)Genomic DNA from 1 ml of blood, mean yield; 30.85 μg, mean purity; 1.82
|
Figure 2. Genomic DNA from 100 μl and 1 ml of human whole blood purified with MagListo™ 5M Genomic DNA Extraction Kit.
 |
M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from 200 μl of blood, mean yield; 6.65 μg, mean purity; 1.84
3; (Maxi)Genomic DNA from 4 ml of blood, yield; 127.5 μg, purity; 1.88
|
Figure 3. Genomic DNA from 200 μl and 4 ml of human whole blood purified with MagListo™ 5M Genomic DNA Extraction Kit.
 |
M; Bioneer 1 kb ladder
1-4; Cell genomic DNA purified with Bioneer MagListo™ 5M Genomic DNA Extraction Kit, mean yield; 24.1 μg, mean purity; 1.92
5-6; Cell genomic DNA purified with competitor Q kit, mean yield; 23.3 μg, mean purity; 2.00
|
Figure 4. Comparison of genomic DNA from HeLa cell (1 x 106) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).
 |
M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from HeLa cell (1x106), mean yield; 13.6 μg, mean purity; 1.83
3-4; (Midi)Genomic DNA from HeLa cell (5x106), mean yield; 75.3 μg, mean purity; 1.83
|
Figure 5. Genomic DNA from 1x106 and 5x106 of HeLa cell purified with MagListo™ 5M Genomic DNA Extraction Kit.
 |
M; Bioneer 1 kb ladder
1-4; Tissue genomic DNA purified with Bioneer MagListo™ 5M Genomic DNA Extraction Kit, mean yield; 5.48 μg, mean purity; 1.86
5-6; Tissue genomic DNA purified with competitor Q kit, mean yield; 5.60 μg, mean purity; 1.85
|
Figure 6. Comparison of genomic DNA from bovine skeletal muscle tissue (15 mg) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).
 |
M; Bioneer 1 kb ladder
1-3; (Mini)Genomic DNA form 20 mg of tissue, mean yield; 5.48 μg, mean purity; 1.81
4-5; (Midi)Genomic DNA form 100 mg of tissue, mean yield; 23.9 μg, mean purity; 1.81
6; (Maxi)Genomic DNA form 250 mg of tissue, yield; 63.4 μg, purity; 1.81
|
Figure 7. Genomic DNA from 20 mg, 100 mg and 250 mg of porcine skeletal muscle tissue purified with MagListo™ 5M Genomic DNA Extraction Kit.
 |
M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from E. coli (1 x 109) purified with Bioneer MagListo™ 5M Genomic DNA Extraction Kit, mean yield; 14.7 μg, mean purity; 1.83
3-4; (Mini)Genomic DNA from E. coli (1 x 109) purified with competitor Q kit, mean yield; 15.5 μg, mean purity; 2.05
5-6; (Midi)Genomic DNA from E. coli (5 x 109), mean yield; 83.9 μg, mean purity; 1.80
7; (Maxi)Genomic DNA from E. coli (1 x 1010), yield; 141.6 μg, purity; 1.78
|
Figure 8. Comparison of genomic DNA from E. coli purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).
Figure 9. Comparison of real-time qPCR data of genomic DNA from HeLa cell (1 x 101 - 1 x 105) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).
A. Amplication curve of GAPDH from HeLa cell (1 x 101 - 1 x 105) genomic DNA purified with Bioneer
MagListo™ 5M Genomic DNA Extraction Kit. The premix used was Accupower® Plus DualStar™ qPCR
PreMix (K-6600).
B. Comparison of standard curves from qPCR results using genomic DNA purified with Bioneer MagListo™
Genomic DNA Extraction Kit and competitor Q kit.
Figure 10. Comparison of DNA extraction time from blood (200 μl) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).
N: Number of sample
Figure 11. Comparison of DNA extraction time from blood purified with MagListo™ 5M Genomic DNA Extraction Kit.
N: Number of sample
