Taq DNA Polymerase

Thermus aquaticus YT1에서 유래된 DNA polymerase gene을 Escherichia coli로부터 발현시킨 후 정제 과정을 거친 내열성의 Taq DNA polymerase입니다.

As low as ₩71,000

카탈로그 번호

제품 개요

특장점

높은 효율/민감도
높은 증폭 효율과 template에 대해 높은 민감도를 갖습니다.
최적화된 버퍼 제공
Enzyme에 최적화된 버퍼를 제공하여 안정적인 PCR 반응을 수행합니다.
재현성
ISO 9001 품질시스템 하에서 생산되어 각 batch에 대한 균일한 품질의 제품이 공급되기 때문에 재현성 있는 결과를 얻을 수 있습니다.

응용 및 적용

Real-time quantification of DNA and cDNA targets using dual probe and dsDNA binding dye
Gene expression profiling
Microbial & viral pathogen detection

실험 자료

Figure 1. Taq DNA Polymerase의 sensitivity test.
Taq DNA Polymerase 1 unit으로 DNA Polymerase의 활성을 확인한 실험 (A) human genomic DNA (B) Lambda DNA

M; 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1; 100 ng template DNA
Lane 2; 10 ng template DNA
Lane 3; 1 ng template DNA
Lane 4; 100 pg template DNA
Lane 5; 10 pg template DNA
Lane 6; 1 pg template DNA
Lane 7; 100 fg template DNA

제품 규격/사양

▶ 제품 규격/사양

5' to 3' exonuclease activity	Yes
3' to 5' exonuclease activity	No
3' – A overhang	Yes
Fragment size	Up to 10 kb

▶ 제품 구성

• 10X reaction buffer with (or without) MgCl₂: Tris-HCl, KCl, 15 mM MgCl₂, pH 9.0

• Dilution buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol, pH 8.0

• 10 mM dNTPs mix: 2.5 mM of each dNTP

▶ 농도

500 units (5 U/μl)

▶ 저장 조건

20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol pH 8.0

▶ 보관 온도

-20°C

▶ 단위(Unit) 정의

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72℃.

▶ 실험 자료

Figure 1. Taq DNA polymerase serial dilution test.Taq DNA Polymerase를 1/5, 1/10, 1/15, 1/20로 희석하여
human genomic DNA (A), Lambda DNA (B) 를 template로 하여 PCR 반응

M; 1 kb DNA Ladder (Cat. No. D-1040, Bioneer)
Lane 1; 5 U of Taq DNA polymerase
Lane 2; 1 U of Taq DNA polymerase
Lane 3; 0.5 U of Taq DNA polymerase
Lane 4; 0.33 U of Taq DNA polymerase
Lane 5; 0.25 U of Taq DNA polymerase

Figure 2. Taq DNA Polymerase의 long kb DNA 증폭 실험.
5 kb 부터 8 kb 까지 Lambda DNA 20 pg을 template로 이용하여 증폭 test.

M; 1 kb DNA Ladder (Cat. No. D-1040, Bioneer)
Lane 1; 5 kb PCR product
Lane 2; 6 kb PCR product
Lane 3; 7 kb PCR product
Lane 4; 8 kb PCR product

◈ Manual

• Manual_Taq DNA Polymerase

• Manual_10X Reaction buffer for Taq DNA polymerase

◈ MSDS

• MSDS_10 mM dNTPs

• MSDS_20 mM MgCl₂

• MSDS_10X Reaction buffer without MgCl2 for Taq DNA polymerase

• MSDS_10X Reaction buffer for Taq DNA polymerase

• MSDS_Dilution buffer for Taq DNA polymerase

• MSDS_Taq DNA polymerase

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

주문 정보

카탈로그 번호	제품명	규격			가격
E-2011	Taq DNA Polymerase	500 U	10X Reaction Buffer with MgCl₂	10 mM dNTP	₩E-2011
E-2011-2			10X Reaction Buffer with MgCl₂		₩E-2011-2
E-2011-1			10X Reaction Buffer, without 20 mM MgCl₂	10 mM dNTP	₩E-2011-1
E-2011-3			10X Reaction Buffer, without 20 mM MgCl₂		₩E-2011-3
E-2013		2,000 U	10X Reaction Buffer with MgCl₂	10 mM dNTP	₩E-2013
E-2013-2			10X Reaction Buffer with MgCl₂		₩E-2013-2
E-2013-1			10X Reaction Buffer, without 20 mM MgCl₂	10 mM dNTP	₩E-2013-1
E-2013-3			10X Reaction Buffer, without 20 mM MgCl₂		₩E-2013-3