Specifications
| Target |
ETEC-Toxin 4 (LT, STa, STb, STX2e) |
| Instrument |
Thermal Cycler(유전자증폭장치) |
| Specimen |
Feces, small and large intestines, septicemia, urine, other systemic organs |
| Time to result |
2 hours (Post-extraction) |
| Tests per Kit |
96 |
Performances
Figure 1. AccuPower® ETEC-Toxin 4-Plex PCR Kit 성능 실험
AccuPower® ETEC-Toxin 4-Plex PCR Kit를 이용하여, 총 4 ng의 Genomic DNA(균주 EcL6601 / EcL7805 각 2 ng)를 95℃ 5 min, 95℃ 30 sec 65℃ 1 min 30 sec(30 cycles), 72℃ 5 min 조건으로 PCR 반응을 진행하였습니다. 3 μl PCR 반응산물을 2% TBE Agarose 젤에서 전기영동하였습니다.
Lane 1: ETEC-Toxin genomic DNA
Lane M: 100bp DNA Ladder (D-1030)
Lane N: Negative control
Figure 2. AccuPower® ETEC-Toxin 4-Plex PCR Kit 민감도 실험 총 4 ng Genomic DNA(균주 EcL6601 / EcL7805 각 2 ng)를 95℃ 5 min, 95℃ 30 sec 65℃ 1 min 30 sec(30 cycles), 72℃ 5 min으로 PCR 반응을 진행하였습니다. PCR 반응산물 3 μl를 2% TBE Agarose 젤에서 전기영동 하였습니다.
Lane 1: 1X10
8 CFU/ml
Lane 2: 1X10
7 CFU/ml
Lane 3: 1X10
6 CFU/ml
Lane 4: 1X10
5 CFU/ml
Lane 5: 1X10
4 CFU/ml
Lane 6: 1X10
3 CFU/ml
Lane 7: 1X10
2 CFU/ml
Lane M: 100bp DNA Ladder(D-1030)
Lane N: Negative control
Figure 5. 장내 유사세균 15종에 대한 AccuPower® ETEC-Toxin 4-Plex PCR Kit 특이도 실험 각각의 장내유사세균 유래 Genomic DNA 4 ng을 95℃ 5 min, 95℃ 30 sec 65℃ 1 min 30 sec(30 cycles), 72℃ 5 min의 조건으로 PCR 반응을 진행하였습니다. 3 µl PCR 반응산물을 2% TBE 젤에서 전기영동하였습니다.
Lane 1:
Acinetobacter lwoffii genomic DNA
Lane 2: Enterococcus fecalis genomic DNA
Lane 3: Maltophilia genomic DNA
Lane 4: Pseudomonas putida genomic DNA
Lane 5: Proteus mirabilis genomic DNA
Lane 6: Citrobacter freundii genomic DNA
Lane 7: Klebsiella pneumonia sub. genomic DNA
Lane 8: Enterobacter cloacae genomic DNA
Lane 9: Acinetobac-ter haemolyticus genomic DNA
Lane 10: Comamonas testosterone genomic DNA
Lane 11: Clostridium perfrigens alpha genomic DNA
Lane 12: Burkholderia cepacia genomic DNA
Lane 13: Ochrobactrum anthropi genomic DNA
Lane 14: Serratia marcescens genomic DNA
Lane 15: Acinetobacter junii genomic DNA
Lane M: 100 bp DNA Ladder (D-1030)
Lane N : Negative control
v 0.0 / 2018-01-01
